Tornado’s Raman Analyzers have been developed with compatibility to a wide range of automated bioreactor systems for cell culture process development and optimization. For example, via the integration to the Sartorius Ambr® systems, or via automated transfer of bioreactor sample data, Tornado Raman Analyzers can return sensitive, accurate analytical results in real-time. Tornado’s Raman Analyzers (HyperFlux™PRO Plus and PGR™) with the RamanBioConnect™ probe measure key process parameters and critical metabolites (i.e., glucose, glutamate, lactate, and others), making it the ideal solution for monitoring bioprocesses and cell cultures.

Additional Studies

In this study (https://www.frontiersin.org/articles/10.3389/fbioe.2022.719614/full), an in-line flow cell for Raman spectroscopy using Tornado’s system is included in the cell-free harvest stream of a perfusion process. Quantitative models for glucose and lactate were generated based on five cultivations originating from varying bioreactor scales. After successfully validating the glucose model, it was employed for full-loop control of an external glucose feed in cultivation with a glucose-free perfusion medium. The generated model was successfully applied to perform process control at 4 g/L and 1.5 g/L glucose over several days, respectively, with variability of ±0.4 g/L. The results demonstrate the high potential of Raman spectroscopy for advanced process monitoring and control of a perfusion process with a bioreactor and scale-independent measurement method.